Host metabolites stimulate the bacterial proton motive force to enhance the activity of aminoglycoside antibiotics.

ABSTRACT: Antibiotic susceptibility of bacterial pathogens is typically evaluated using in vitro assays that do not consider the complex host microenvironment. This may help explaining a significant discrepancy between antibiotic efficacy in vitro and in vivo, with some antibiotics being effective in vitro but not in vivo or vice versa. Nevertheless, it is well-known that antibiotic susceptibility of bacteria is driven by environmental factors. Lung epithelial cells enhance the activity of aminoglycoside antibiotics against the opportunistic pathogen Pseudomonas aeruginosa, yet the mechanism behind is unknown. The present study addresses this gap and provides mechanistic understanding on how lung epithelial cells stimulate aminoglycoside activity. To investigate the influence of the local host micro

SUBMITTER: Crabbe A 

PROVIDER: S-EPMC6508747 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature