Development of an activation tagging system for maize.

ABSTRACT: Activation Tagging, distributing transcriptional enhancers throughout the genome to induce transcription of nearby genes, is a powerful tool for discovering the function of genes in plants. We have developed a transposable element system to distribute a novel activation tagging element throughout the genome of maize. The transposon system is built from the Enhancer/Suppressor (En/Spm) transposon system and uses an engineered seed color marker to show when the transposon excises. Both somatic and germinal excision events can be detected by the seed color. The activation tagging element is in a Spm-derived non-autonomous transposon and contains four copies of the Sugarcane Bacilliform Virus-enhancer (SCBV-enhancer) and the AAD1 selectab

SUBMITTER: Davies JP 

PROVIDER: S-EPMC6508757 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature