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The Influenza A Virus M2 Protein trans-Complementation System Offers a Set of Tools for the Undergraduate Virology Laboratory.

ABSTRACT: An authentic, hands-on experience in the laboratory is an important part of any undergraduate biology course. However, there are a limited number of mammalian virus systems that students can work with safely in an undergraduate teaching laboratory. For many systems, the risk to the students is too high. The influenza A virus M2 protein trans-complementation system bridges this gap. This system consists of a virus with mutations that prevent the expression of the essential M2 protein; therefore this virus can only replicate in a cell line that provides M2 in trans. Here, we describe the use of this system to carry out hemagglutination, real-time reverse transcriptase PCR, 50% tissue culture infectious dose, and plaque assays in an undergraduate lab setting.

SUBMITTER: Grantham ML

PROVIDER: S-EPMC6508898 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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The Influenza A Virus M2 Protein <i>trans</i>-Complementation System Offers a Set of Tools for the Undergraduate Virology Laboratory.

Grantham Michael L ML McCown Matthew F MF Pekosz Andrew A

Journal of microbiology & biology education 20190426 1

An authentic, hands-on experience in the laboratory is an important part of any undergraduate biology course. However, there are a limited number of mammalian virus systems that students can work with safely in an undergraduate teaching laboratory. For many systems, the risk to the students is too high. The influenza A virus M2 protein <i>trans</i>-complementation system bridges this gap. This system consists of a virus with mutations that prevent the expression of the essential M2 protein; ther ...[more]

PMID: 31160927

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