Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging.

ABSTRACT: Recent advances in super-resolution microscopy allow the localization of single molecules within individual cells but not within multiple whole cells due to weak signals from single molecules and slow acquisition process for point accumulation to reconstruct super-resolution images. Here, we report a fast, large-scale, and three-dimensional super-resolution fluorescence microscope based on single-wavelength Bessel lightsheet to selectively illuminate spontaneous blinking fluorophores tagged to the proteins of interest in space. Critical parameters such as labeling density, excitation power, and exposure time were systematically optimized resulting in a maximum imaging speed of 2.7 × 10⁴ µm³ s^-1. Fourier ring correlation analysis revealed a reconstructed ima

SUBMITTER: Lu CH 

PROVIDER: S-EPMC6509110 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature