Targeted removal of epigenetic barriers during transcriptional reprogramming.

ABSTRACT: Master transcription factors have the ability to direct and reverse cellular identities, and consequently their genes must be subject to particular transcriptional control. However, it is unclear which molecular processes are responsible for impeding their activation and safeguarding cellular identities. Here we show that the targeting of dCas9-VP64 to the promoter of the master transcription factor Sox1 results in strong transcript and protein up-regulation in neural progenitor cells (NPCs). This gene activation restores lost neuronal differentiation potential, which substantiates the role of Sox1 as a master transcription factor. However, despite efficient transactivator binding, major proportions of progenitor cells are unresponsive to the transactivating stimulus. By combining the tran

SUBMITTER: Baumann V 

PROVIDER: S-EPMC6509258 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature