Genetic transformation of tropical maize (Zea mays L.) inbred line with a phytase gene from Aspergillus niger.

ABSTRACT: A full-length cDNA of phyA gene of Aspergillus niger, encoding phytase enzyme, was cloned and expressed in E. coli BL21 cells and assayed for its activity. The phyA cDNA consisted of 1404 bp, which encoded 467 amino acid residues. The phytase activity of purified phytase was 826.33 U/mL. The phyA gene under the control of endosperm-specific promoters was transformed into an Indian maize inbred line, UMI29, using particle bombardment-mediated transformation method to generate transgenic maize plants over-expressing phytase in seeds. PCR and GUS analyses demonstrated the presence of transgenes in T₀ transgenic plants and their stable inheritance in the T₁ progenies. Three transgenic events expressing detectable level of A. niger phytas

SUBMITTER: Geetha S 

PROVIDER: S-EPMC6509297 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature