Properties of the HtrA Protease From Bacterium Helicobacter pylori Whose Activity Is Indispensable for Growth Under Stress Conditions.

ABSTRACT: The protease high temperature requirement A from the gastric pathogen Helicobacter pylori (HtrA _Hp ) belongs to the well conserved family of serine proteases. HtrA _Hp is an important secreted virulence factor involved in the disruption of tight and adherens junctions during infection. Very little is known about the function of HtrA _Hp in the H. pylori cell physiology due to the lack of htrA knockout strains. Here, using a newly constructed ΔhtrA mutant strain, we found that bacteria deprived of HtrA _Hp showed increased sensitivity to certain types of stress, including elevated temperature, pH and osmotic shock, as well as treatment with puromycin. These data indicate that HtrA _Hp

SUBMITTER: Zarzecka U 

PROVIDER: S-EPMC6509562 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature