Transcriptome and physiological analyses for revealing genes involved in wheat response to endoplasmic reticulum stress.

ABSTRACT:

Background

Wheat production is largely restricted by adverse environmental stresses. Under many undesirable conditions, endoplasmic reticulum (ER) stress can be induced. However, the physiological and molecular responses of wheat to ER stress remain poorly understood. We used dithiothreitol (DTT) and tauroursodeoxycholic acid (TUDCA) to induce or suppress ER stress in wheat cells, respectively, with the aim to reveal the molecular background of ER stress responses using a combined approach of transcriptional profiling and morpho-physiological characterization.

Methods

To understand the mechanism of wheat response to ER stress, three wheat cultivars were used in our pre-experiments. Among them, the cultivar with a moderate stress tolerance, Yunong211 was used in the following

SUBMITTER: Yu X 

PROVIDER: S-EPMC6509841 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature