IR-783 Labeling of a Peptide Receptor for 'Turn-On' Fluorescence Based Sensing.

ABSTRACT: In this study, we examine a means for developing near-IR fluorescent sensors through streamlined, site-specific coupling with peptide-based receptors. As the penultimate step of solid-phase synthesis of a peptide-based receptor, we show a simple means of labeling the N' terminus with the near IR fluorophore IR-783 to afford a viable fluorescent sensor after cleavage from the resin. The proof-of-concept probe utilized a biotin mimetic peptide sequence as the receptive moiety. Here we revealed a "turn-on" fluorescence enhancement upon binding of the biotin mimetic probe to its intended streptavidin target. Not all peptide-receptive moieties tested were able to generate such an enhancement upon target binding, and as such, the rationale for the observed fluorescence response properties is dis

SUBMITTER: Kwak EA 

PROVIDER: S-EPMC6510487 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature