How Outer Coordination Sphere Modifications Can Impact Metal Structures in Proteins: A Crystallographic Evaluation.

ABSTRACT: A challenging objective of de novo metalloprotein design is to control of the outer coordination spheres of an active site to fine tune metal properties. The well-defined three stranded coiled coils, TRI and CoilSer peptides, are used to address this question. Substitution of Cys for Leu yields a thiophilic site within the core. Metals such as Hg^II , Pb^II , and As^III result in trigonal planar or trigonal pyramidal geometries; however, spectroscopic studies have shown that Cd^II forms three-, four- or five-coordinate Cd^II S₃ (OH₂ )_x (in which x=0-2) when the outer coordination spheres are perturbed. Unfortunately, there has been little crystallographic examination of these proteins to explain the observat

SUBMITTER: Ruckthong L 

PROVIDER: S-EPMC6510599 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature