Human adipose stromal-vascular fraction self-organizes to form vascularized adipose tissue in 3D cultures.

ABSTRACT: Native human subcutaneous adipose tissue (AT) is well organized into unilocular adipocytes interspersed within dense vascularization. This structure is completely lost under standard culture conditions and may impair the comparison with native tissue. Here, we developed a 3-D model of human white AT reminiscent of the cellular architecture found in vivo. Starting with adipose progenitors derived from the stromal-vascular fraction of human subcutaneous white AT, we generated spheroids in which endogenous endothelial cells self-assembled to form highly organized endothelial networks among stromal cells. Using an optimized adipogenic differentiation medium to preserve endothelial cells, we obtained densely vascularized spheroids containing mature adipocytes with unilocular lipid vacuoles. In

SUBMITTER: Muller S 

PROVIDER: S-EPMC6510792 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature