PTrimmer: An efficient tool to trim primers of multiplex deep sequencing data.

ABSTRACT:

Background

With the widespread use of multiple amplicon-sequencing (MAS) in genetic variation detection, an efficient tool is required to remove primer sequences from short reads to ensure the reliability of downstream analysis. Although some tools are currently available, their efficiency and accuracy require improvement in trimming large scale of primers in high throughput target genome sequencing. This issue is becoming more urgent considering the potential clinical implementation of MAS for processing patient samples. We here developed pTrimmer that could handle thousands of primers simultaneously with greatly improved accuracy and performance.

Result

pTrimmer combines the two algorithms of k-mers and Needleman-Wunsch algorithm, which ensures its accuracy even with the p

SUBMITTER: Zhang X 

PROVIDER: S-EPMC6511130 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature