Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis.

ABSTRACT:

Background

Canine parvovirus (CPV) and feline parvovirus (FPV) are causative agents of diarrhea in dogs and cats, which manifests as depression, vomiting, fever, loss of appetite, leucopenia, and diarrhea in young animals. CPV and FPV can single or mixed infect cats and cause disease. To diagnose sick animals effectively, an effective virus diagnostic and genome typing method with high sensitivity and specificity is required.

Results

In this study, a conserved segment containing one SNP A4408C of parvovirus was used for real-time PCR amplification. Subsequently, data were auto-analyzed and plotted using Applied Biosystems® High Resolution Melt Software v3.1. Results showed that CPV and FPV can be detected simultaneously in a single PCR reaction. No cross-reactions were obser

SUBMITTER: Sun Y 

PROVIDER: S-EPMC6511188 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature