Structural and Functional Studies of Bacterial Enolase, a Potential Target against Gram-Negative Pathogens.

ABSTRACT: Enolase is a glycolytic metalloenzyme involved in carbon metabolism. The advantage of targeting enolase lies in its essentiality in many biological processes such as cell wall formation and RNA turnover and as a plasminogen receptor. We initially used a DARTS assay to identify enolase as a target in Escherichia coli. The antibacterial activities of α-, β-, and γ-substituted seven-member ring tropolones were first evaluated against four strains representing a range of Gram-negative bacteria. We observed that the chemical properties and position of the substituents on the tropolone ring play an important role in the biological activity of the investigated compounds. Both α- and β-substituted phenyl derivatives of tropolone were the most active with minimum inhibitory concentrations in the ra

SUBMITTER: Krucinska J 

PROVIDER: S-EPMC6511404 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature