Next-level riboswitch development-implementation of Capture-SELEX facilitates identification of a new synthetic riboswitch.

ABSTRACT: The development of synthetic riboswitches has always been a challenge. Although a number of interesting proof-of-concept studies have been published, almost all of these were performed with the theophylline aptamer. There is no shortage of small molecule-binding aptamers; however, only a small fraction of them are suitable for RNA engineering since a classical SELEX protocol selects only for high-affinity binding but not for conformational switching. We now implemented RNA Capture-SELEX in our riboswitch developmental pipeline to integrate the required selection for high-affinity binding with the equally necessary RNA conformational switching. Thus, we successfully developed a new paromomycin-binding synthetic riboswitch. It binds paromomycin with a KD of 20 nM and can discriminate between

SUBMITTER: Boussebayle A 

PROVIDER: S-EPMC6511860 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature