Growth suppression by altered (p)ppGpp levels results from non-optimal resource allocation in Escherichia coli.

ABSTRACT: Understanding how bacteria coordinate gene expression with biomass growth to adapt to various stress conditions remains a grand challenge in biology. Stress response is often associated with dramatic accumulation of cellular guanosine tetra- or penta-phosphate (p)ppGpp (also known as 'magic spot'), which is a key second messenger participating in regulating various biochemical and physiological processes of bacteria. Despite of the extensive studies on the mechanism of gene regulation by (p)ppGpp during stringent response, the connection between (p)ppGpp and bacterial steady-state exponential growth remains elusive. Here, we establish a versatile genetic approach to systematically perturb the (p)ppGpp level of Escherichia coli through titrating either the single-function (p)ppGpp synthetas

SUBMITTER: Zhu M 

PROVIDER: S-EPMC6511861 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature