Invadopodia biogenesis is regulated by caveolin-mediated modulation of membrane cholesterol levels.

ABSTRACT: Invadopodia are proteolytically active protrusions formed by invasive tumoural cells when grown on an extracellular matrix (ECM) substratum. Clearly, invadopodia are specialized membrane domains acting as sites of signal transduction and polarized delivery of components required for focalized ECM degradation. For these reasons, invadopodia are a model to study focal ECM degradation by tumour cells. We investigated the features of invadopodia membrane domains and how altering their composition would affect invadopodia biogenesis and function. This was achieved through multiple approaches including manipulation of the levels of cholesterol and other lipids at the plasma membrane, alteration of cholesterol trafficking by acting on caveolin 1 expression and phosphorylation. We show that choles

SUBMITTER: Caldieri G 

PROVIDER: S-EPMC6512369 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature