Determination of polycyclic aromatic hydrocarbons with molecular mass 302 in Standard Reference Material 1597a by reversed-phase liquid chromatography and stop-flow fluorescence detection.

ABSTRACT: The identification of isomeric polycyclic aromatic hydrocarbons (PAHs) in complex samples via reversed-phase liquid chromatography (RPLC) with fluorescence detection (FL) is normally based on matching the chromatographic retention times of suspected peaks of interest with reference standards. Since no spectral information is obtained during the chromatographic run, the accurate identification of co-eluting PAHs with similar chromatographic behaviors requires confirmation with additional chromatographic methods. This is particularly true for the analysis of PAH isomers with the relative molecular mass (MM, g/mol) 302. The work presented here explores the information content of room-temperature fluorescence spectra for the analysis of PAHs with MM 302 in the Standard Reference Material (SRM)

SUBMITTER: Hayes HV 

PROVIDER: S-EPMC6512832 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature