Plasmid engineering of aphid alarm pheromone in tobacco seedlings affects the preference of aphids.

ABSTRACT: Plants producing sufficient amount of aphid alarm pheromone by expressing (E)-β-Farnesene (EβF) synthase gene may contribute to plant protection by reducing aphid populations. However, terpene biosynthesis varies among plant species and developmental stages. In the present study, volatile headspace analysis of tobacco seedlings with MaβFS1 (an EβF synthase from the Asian peppermint Mentha asiatica) failed to generate EβF. We further targeted MaβFS1 to the tobacco plastid, using a chloroplast targeting sequence, either with or without the AtFPS1 gene for the biosynthesis of the precursor farnesyl diphosphate. When both MaβFS1 and AtFPS1 genes were targeted to the chloroplast, low levels of EβF were detected in stably transformed tobacco seedlings; resulting in specific repellence of the green peach aphid, Myzus persicae. These data indicate that redirecting the EβF biosynthetic pathway from its natural cytosolic location to the chloroplast is a valid strategy. This redirecting strategy may be very useful for other crop plants that do not naturally produce EβF or other repellent volatiles.

SUBMITTER: Yu X 

PROVIDER: S-EPMC6512937 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature