Knockdown of PTOV1 and PIN1 exhibit common phenotypic anti-cancer effects in MDA-MB-231 cells.

ABSTRACT:

Background

Earlier, we have identified PTOV1 as a novel interactome of PIN1 in PC-3 cells. This study aims to explore the functional similarity and the common role of both genes in breast cancer cell proliferation.

Methods

CTG, crystal violet assay, clonogenic assay, wound healing assay, cell cycle analysis, Hoechst staining and ROS measurement were performed to assess cell viability, colony forming potential, cell cycle arrest, nuclear condensation and ROS production after knocking down of PTOV1 and PIN1 by siRNAs in MDA-MB-231 and MCF-7 cells. CO-IP, qPCR and western blot were performedto study interaction, transcriptional and translational regulation of both genes.

Results

Knockdown of PTOV1 and PIN1 inhibited the cell proliferation, colony formation, migration, ce

SUBMITTER: Karna SKL 

PROVIDER: S-EPMC6513092 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature