Quantitative 3D Mapping of the Human Skeletal Muscle Mitochondrial Network.

ABSTRACT: Genetic and biochemical defects of mitochondrial function are a major cause of human disease, but their link to mitochondrial morphology in situ has not been defined. Here, we develop a quantitative three-dimensional approach to map mitochondrial network organization in human muscle at electron microscopy resolution. We establish morphological differences between human and mouse and among patients with mitochondrial DNA (mtDNA) diseases compared to healthy controls. We also define the ultrastructure and prevalence of mitochondrial nanotunnels, which exist as either free-ended or connecting membrane protrusions across non-adjacent mitochondria. A multivariate model integrating mitochondrial volume, morphological complexity, and branching anisotropy computed across individual mitochondria an

SUBMITTER: Vincent AE 

PROVIDER: S-EPMC6513570 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature