Functional and molecular characterization of the conserved Arabidopsis PUMILIO protein, APUM9.

ABSTRACT:

Key message

Here we demonstrate that the APUM9 RNA-binding protein and its co-factors play a role in mRNA destabilization and how this activity might regulate early plant development. APUM9 is a conserved PUF RNA-binding protein (RBP) under complex transcriptional control mediated by a transposable element (TE) that restricts its expression in Arabidopsis. Currently, little is known about the functional and mechanistic details of the plant PUF regulatory system and the biological relevance of the TE-mediated repression of APUM9 in plant development and stress responses. By combining a range of transient assays, we show here, that APUM9 binding to target transcripts can trigger their rapid decay via its conserved C-terminal RNA-binding domain. APUM9 directly interacts with DCP2, the

SUBMITTER: Nyiko T 

PROVIDER: S-EPMC6513901 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature