A Device for measuring the in-situ response of Human Bronchial Epithelial Cells to airborne environmental agents.

ABSTRACT: Measuring the time evolution of response of Normal Human Bronchial Epithelial (NHBE) cells to aerosols is essential for understanding the pathogenesis of airway disease. This study introduces a novel Real-Time Examination of Cell Exposure (RTECE) system, which enables direct in situ assessment of functional responses of the cell culture during and following exposure to environmental agents. Included are cell morphology, migration, and specialised responses, such as ciliary beat frequency (CBF). Utilising annular nozzles for aerosol injection and installing windows above and below the culture, the cells can be illuminated and examined during exposure. The performance of RTECE is compared to that of the commercial Vitrocell by exposing NHBE cells to cigarette smoke. Both systems show the sam

SUBMITTER: Chandrala LD 

PROVIDER: S-EPMC6513995 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature