Divergent Aging of Isogenic Yeast Cells Revealed through Single-Cell Phenotypic Dynamics.

ABSTRACT: Although genetic mutations that alter organisms' average lifespans have been identified in aging research, our understanding of the dynamic changes during aging remains limited. Here, we integrate single-cell imaging, microfluidics, and computational modeling to investigate phenotypic divergence and cellular heterogeneity during replicative aging of single S. cerevisiae cells. Specifically, we find that isogenic cells diverge early in life toward one of two aging paths, which are characterized by distinct age-associated phenotypes. We captured the dynamics of single cells along the paths with a stochastic discrete-state model, which accurately predicts both the measured heterogeneity and the lifespan of cells on each path within a cell population. Our analysis suggests that genetic and env

SUBMITTER: Jin M 

PROVIDER: S-EPMC6514117 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature