Molecular mechanisms of caffeine-mediated intestinal epithelial ion transports.

ABSTRACT:

Background and purpose

As little is known about the effect of caffeine, one of the most widely consumed substances worldwide, on intestinal function, we aimed to study its action on intestinal anion secretion and the underlying molecular mechanisms.

Experimental approach

Anion secretion and channel expression were examined in mouse duodenal epithelium by Ussing chambers and immunocytochemistry. Ca²⁺ imaging was also performed in intestinal epithelial cells (IECs).

Key results

Caffeine (10 mM) markedly increased mouse duodenal short-circuit current (I_sc ), which was attenuated by a removal of either Cl^- or HCO₃ ^- , Ca²⁺ -free serosal solutions and selective blockers of store-operated Ca²⁺ chan

SUBMITTER: Zhang F 

PROVIDER: S-EPMC6514291 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature