Protein inhibitor of activated STAT1 Ser⁵⁰³ phosphorylation-mediated Elk-1 SUMOylation promotes neuronal survival in APP/PS1 mice.

ABSTRACT:

Background and purpose

Protein inhibitor of activated STAT1 (PIAS1) is phosphorylated by IKKα at Ser⁹⁰ in a PIAS1 E3 ligase activity-dependent manner. Whether PIAS1 is also phosphorylated at other residues and the functional significance of these additional phosphorylation events are not known. The transcription factor Elk-1 remains SUMOylated under basal conditions, but the role of Elk-1 SUMOylation in brain is unknown. Here, we examined the functional significance of PIAS1-mediated Elk-1 SUMOylation in Alzheimer's disease (AD) using the APP/PS1 mouse model of AD and amyloid β (Aβ) microinjections in vivo.

Experimental approach

Novel phosphorylation site(s) on PIAS1 were identified by LC-MS/MS, and MAPK/ERK-mediated phosphorylation of Elk-1 demonstrated using in

SUBMITTER: Liu SY 

PROVIDER: S-EPMC6514297 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature