Hypoxia modulates protein phosphatase 2A through HIF-1α dependent and independent mechanisms in human aortic smooth muscle cells and ventricular cardiomyocytes.

ABSTRACT:

Background and purpose

Although protein phosphatases regulate multiple cellular functions, their modulation under hypoxia remains unclear. We investigated expression of the protein phosphatase system under normoxic/hypoxic conditions and the mechanism by which hypoxia alters protein phosphatase 2A (PP2A) activity.

Experimental approach

Human cardiovascular cells were cultured in cell type specific media under normoxic or hypoxic conditions (1% O₂ ). Effects on mRNA expression, phosphatase activity, post-translational modification, and involvement of hypoxia inducible factor 1α (HIF-1α) were assessed using RT-PCR, immunoblotting, an activity assay, and siRNA silencing.

Key results

All components of the protein phosphatase system studied were expressed in eac

SUBMITTER: Elgenaidi IS 

PROVIDER: S-EPMC6514298 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature