Cellular Metabolic Heterogeneity In Vivo Is Recapitulated in Tumor Organoids.

ABSTRACT: Heterogeneous populations within a tumor have varying metabolic profiles, which can muddle the interpretation of bulk tumor imaging studies of treatment response. Although methods to study tumor metabolism at the cellular level are emerging, these methods provide a single time point "snapshot" of tumor metabolism and require a significant time and animal burden while failing to capture the longitudinal metabolic response of a single tumor to treatment. Here, we investigated a novel method for longitudinal, single-cell tracking of metabolism across heterogeneous tumor cell populations using optical metabolic imaging (OMI), which measures autofluorescence of metabolic coenzymes as a report of metabolic activity. We also investigated whether in vivo cellular metabolic heterogeneity can be acc

SUBMITTER: Sharick JT 

PROVIDER: S-EPMC6514366 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature