Proteolytic dynamics of human 20S thymoproteasome.

ABSTRACT: An efficient immunosurveillance of CD8⁺ T cells in the periphery depends on positive/negative selection of thymocytes and thus on the dynamics of antigen degradation and epitope production by thymoproteasome and immunoproteasome in the thymus. Although studies in mouse systems have shown how thymoproteasome activity differs from that of immunoproteasome and strongly impacts the T cell repertoire, the proteolytic dynamics and the regulation of human thymoproteasome are unknown. By combining biochemical and computational modeling approaches, we show here that human 20S thymoproteasome and immunoproteasome differ not only in the proteolytic activity of the catalytic sites but also in the peptide transport. These differences impinge upon the quantity of peptide products rather than

SUBMITTER: Kuckelkorn U 

PROVIDER: S-EPMC6514615 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature