A Biosensor Platform for Metal Detection Based on Enhanced Green Fluorescent Protein.

ABSTRACT: Microbial cell-based biosensors, which mostly rely on stress-responsive operons, have been widely developed to monitor environmental pollutants. Biosensors are usually more convenient and inexpensive than traditional instrumental analyses of environmental pollutants. However, the targets of biosensors are restricted by the limited number of genetic operon systems available. In this study, we demonstrated a novel strategy to overcome this limitation by engineering an enhanced green fluorescent protein (eGFP). It has been reported that combining two fragments of split-eGFP can form a native structure. Thus, we engineered new biosensors by inserting metal-binding loops (MBLs) between β-strands 9 and 10 of the eGFP, which then undergoes conformational changes upon interaction between the MBLs

SUBMITTER: Lee W 

PROVIDER: S-EPMC6514868 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature