Identifying Reducing and Capping Sites of Protein-Encapsulated Gold Nanoclusters.

ABSTRACT: The reducing and capping sites along with their local structure impact photo properties of the red bovine serum albumin-capped Au nanocluster (BSA-AuNC), however, they are hard to identify. We developped a workflow and relevant techniques using mass spectrometry (MS) to identify the reducing and capping sites of BSA-AuNCs involved in their formation and fluorescence. Digestion without disulfide cleavages yielded an Au core fraction exhibiting red fluorescence and [Au_nS_m] ion signals and a non-core fraction exhibiting neither of them. The core fraction was identified to mainly be comprised of peptides containing cysteine residues. The fluorescence and [Au_nS_m] signals were quenched by tris(2-carboxyethyl)phosphine, confi

SUBMITTER: Hsu YC 

PROVIDER: S-EPMC6514900 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature