Comparison of macronutrient content in human milk measured by mid-infrared human milk analyzer and reference methods.

ABSTRACT:

Objective

The study aims at evaluating mid-infrared human milk analyzer (HMA) accuracy and precision, in human milk (HM).

Study design

Röse-Gottlieb, high-performance anion exchange chromatography-pulsed amperometric detection (HPAEC-PAD), Kjeldahl and amino acid analysis (AA) were selected as references for total fat, lactose and total protein determination.

Results

No significant difference was observed in lactose content between HMA and HPAEC-PAD. Significant differences were observed in fat and protein content between HMA and reference methods. However, the difference in fat content was lower than 12%, and therefore within the variability declared by supplier. For protein determination, the BCA protein assay was selected. No significant differences were observed i

SUBMITTER: Giuffrida F 

PROVIDER: S-EPMC6514977 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature