A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand.

ABSTRACT:

Background

The Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An. barbirostris, An. campestris, An. dissidens, An. saeungae and An. wejchoochotei, while An. vanderwulpi occurs in Indonesia. As these species cannot be accurately differentiated by morphological characters, there is a crucial lack of information on their bionomics and role in the transmission of malaria and filariasis agents.

Results

For differentiating the six species, an allele-specific amplification (AS-PCR) based on the second internal transcribed spacer (ITS2) sequence was developed.

SUBMITTER: Brosseau L 

PROVIDER: S-EPMC6515612 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature