Exercise activates lysosomal function in the brain through AMPK-SIRT1-TFEB pathway.

ABSTRACT:

Aim

To study the effects of exercise on lysosomal functions.

Methods

Mouse exercise model was established and wheel running was scheduled as 18 rpm (14:00-17:00), 5 d/wk, for 8 weeks. Mice were injected EX527 to inhibit SIRT1 activity. The protein level was assayed with Western blot and immunofluorescence histochemistry. The transmission electron microscopic examination was used to show the structure of lysosome and mitochondria.

Results

Exercise promoted the nuclear translocation of TFEB in the cortex which upregulated the transcription of genes associated with autophagy and lysosome. Exercise directly activated autophagy/lysosome system via up-regulating of AMPK-SIRT1 signaling. The SIRT1 inhibitor EX527 decreased TFEB regulated gene transcription but had little eff

SUBMITTER: Huang J 

PROVIDER: S-EPMC6515701 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature