Abnormal composition of gut microbiota contributes to delirium-like behaviors after abdominal surgery in mice.

ABSTRACT:

Aims

Anesthesia and surgery can cause delirium-like symptoms postoperatively. Increasing evidence suggests that gut microbiota is a physiological regulator of the brain. Herein, we investigated whether gut microbiota plays a role in postoperative delirium (POD).

Methods

Mice were separated into non-POD and POD phenotypes after abdominal surgery by applying hierarchical clustering analysis to behavioral tests. Fecal samples were collected, and 16S ribosomal RNA gene sequencing was performed to detect differences in gut microbiota composition among sham, non-POD, and POD mice. Fecal bacteria from non-POD and POD mice were transplanted into antibiotics-induced pseudo-germ-free mice to investigate the effects on behaviors.

Results

α-diversity and β-diversity indicated dif

SUBMITTER: Zhang J 

PROVIDER: S-EPMC6515708 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature