GPSM2-GNAI Specifies the Tallest Stereocilia and Defines Hair Bundle Row Identity.

ABSTRACT: The transduction compartment of inner ear hair cells, the hair bundle, is composed of stereocilia rows of graded height, a property essential for sensory function that remains poorly understood at the molecular level. We previously showed that GPSM2-GNAI is enriched at stereocilia distal tips and required for their postnatal elongation and bundle morphogenesis-two characteristics shared with MYO15A (short isoform), WHRN, and EPS8 proteins. Here we first performed a comprehensive genetic analysis of the mouse auditory epithelium to show that GPSM2, GNAI, MYO15A, and WHRN operate in series within the same pathway. To understand how these functionally disparate proteins act as an obligate complex, we then systematically analyzed their distribution in normal and mutant bundles over time. We di

SUBMITTER: Tadenev ALD 

PROVIDER: S-EPMC6516530 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature