Silicon µPCR Chip for Forensic STR Profiling with Hybeacon Probe Melting Curves.

ABSTRACT: The demand to perform forensic DNA profiling outside of centralized laboratories and on the crime scene is increasing. Several criminal investigations would benefit tremendously from having DNA based information available in the first hours rather than days or weeks. However, due to the complexity and time-consuming nature of standard DNA fingerprinting methods, rapid and automated analyses are hard to achieve. We here demonstrate the implementation of an alternative DNA fingerprinting method in a single microchip. By combining PCR amplification and HyBeacon melting assays in a silicon Lab-on-a-chip (LoC), a significant step towards rapid on-site DNA fingerprinting is taken. The small form factor of a LoC reduces reagent consumption and increases portability. Additional miniaturization is

SUBMITTER: Cornelis S 

PROVIDER: S-EPMC6517373 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature