Characteristic differences of cell sheets composed of mesenchymal stem cells with different tissue origins.

ABSTRACT:

Introduction

Stem cell therapy with mesenchymal stem cells (MSCs) has been widely used in many clinical trials, and therapy with MSC sheets shows promise for patients. However, there are few reports characterizing MSC sheets. In the present study, the properties of MSC sheets derived from bone marrow, adipose tissue, and umbilical cord were evaluated.

Methods

Cell sheets were fabricated with MSCs from different tissue origins in temperature-responsive cell culture dishes with and without pre-coating of fetal bovine serum (FBS). MSC adhesion behavior in the culture dish was observed. Secretion of cytokines related to cell proliferation and immune regulation from MSC sheets was investigated by ELISA. The adhesion properties of the MSC sheets were investigated by time-lapse mic

SUBMITTER: Nakao M 

PROVIDER: S-EPMC6517796 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature