A beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion in mice.

ABSTRACT:

Aims/hypothesis

To directly assess the role of beta cell lipolysis in insulin secretion and whole-body energy homeostasis, inducible beta cell-specific adipose triglyceride lipase (ATGL)-deficient (B-Atgl-KO) mice were studied under normal diet (ND) and high-fat diet (HFD) conditions.

Methods

Atgl ^flox/flox mice were cross-bred with Mip-Cre-ERT mice to generate Mip-Cre-ERT^/+;Atgl ^flox/flox mice. At 8 weeks of age, these mice were injected with tamoxifen to induce deletion of beta cell-specific Atgl (also known as Pnpla2), and the mice were fed an ND or HFD.

Results

ND-fed male B-Atgl-KO mice showed decreased insulinaemia and glucose-induced insulin secretion (GSIS) in vivo. Changes in GSIS correlated with the islet content of long-chai

SUBMITTER: Attane C 

PROVIDER: S-EPMC6518076 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature