Whole genome sequencing for improved understanding of Mycobacterium tuberculosis transmission in a remote circumpolar region.

ABSTRACT: Few studies have used genomic epidemiology to understand tuberculosis (TB) transmission in rural and remote settings - regions often unique in history, geography and demographics. To improve our understanding of TB transmission dynamics in Yukon Territory (YT), a circumpolar Canadian territory, we conducted a retrospective analysis in which we combined epidemiological data collected through routine contact investigations with clinical and laboratory results. Mycobacterium tuberculosis isolates from all culture-confirmed TB cases in YT (2005-2014) were genotyped using 24-locus Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats (MIRU-VNTR) and compared to each other and to those from the neighbouring province of British Columbia (BC). Whole genome sequencing (WGS)

SUBMITTER: Guthrie JL 

PROVIDER: S-EPMC6518594 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature