Finely-tuned regulation of AMP-activated protein kinase is crucial for human adult erythropoiesis.

ABSTRACT: AMP-activated protein kinase (AMPK) is a heterotrimeric complex containing α, β, and γ subunits involved in maintaining integrity and survival of murine red blood cells. Indeed, Ampk α1^-/- , Ampk β1^-/- and Ampk γ1^-/- mice develop hemolytic anemia and the plasma membrane of their red blood cells shows elasticity defects. The membrane composition evolves continuously along erythropoiesis and during red blood cell maturation; defects due to the absence of Ampk could be initiated during erythropoiesis. We, therefore, studied the role of AMPK during human erythropoiesis. Our data show that AMPK activation had two distinct phases in primary erythroblasts. The phosphorylation of AMPK (Thr172) and its target acetyl CoA carboxylase (Ser79

SUBMITTER: Ladli M 

PROVIDER: S-EPMC6518903 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature