PAD4-dependent NETs generation are indispensable for intestinal clearance of Citrobacter rodentium.

ABSTRACT: Peptidyl arginine deiminase-4 (PAD4) is indispensable for generation of neutrophil extracellular traps (NETs), which can provide antimicrobial effects during host innate immune response; however, the role of PAD4 against gastrointestinal infection is largely unknown. Herein, we challenged PAD4-deficient (Pad4^-/-) mice and wild-type (WT) littermates with Citrobacter rodentium (CR), and investigated bacteria clearance and gut pathology. Luminal colonization of CR in Pad4^-/- mice peaked between 11-14 days post-infection, whereas WT mice suppressed the infection by 14 days. We demonstrated that Pad4^-/- mice were unable to form NETs, whereas WT mice showed increased NETs formation in the colon during infection. Pad4^-/- mice showed aggravated CR-associ

SUBMITTER: Saha P 

PROVIDER: S-EPMC6519124 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature