Comparative study of cerebrospinal fluid α-synuclein seeding aggregation assays for diagnosis of Parkinson's disease.

ABSTRACT:

Background

PD diagnosis is based primarily on clinical criteria and can be inaccurate. Biological markers, such as α-synuclein aggregation, that reflect ongoing pathogenic processes may increase diagnosis accuracy and allow disease progression monitoring. Though α-synuclein aggregation assays have been published, reproducibility, standardization, and validation are key challenges for their development as clinical biomarkers.

Objective

To cross-validate two α-synuclein seeding aggregation assays developed to detect pathogenic oligomeric α-synuclein species in CSF using samples from the same PD patients and healthy controls from the BioFIND cohort.

Methods

CSF samples were tested by two independent laboratories in a blinded fashion. BioFIND features standardized biospec

SUBMITTER: Kang UJ 

PROVIDER: S-EPMC6519150 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature