The efficacy of whole human genome capture on ancient dental calculus and dentin.

ABSTRACT:

Objectives

Dental calculus is among the richest known sources of ancient DNA in the archaeological record. Although most DNA within calculus is microbial, it has been shown to contain sufficient human DNA for the targeted retrieval of whole mitochondrial genomes. Here, we explore whether calculus is also a viable substrate for whole human genome recovery using targeted enrichment techniques.

Materials and methods

Total DNA extracted from 24 paired archaeological human dentin and calculus samples was subjected to whole human genome enrichment using in-solution hybridization capture and high-throughput sequencing.

Results

Total DNA from calculus exceeded that of dentin in all cases, and although the proportion of human DNA was generally lower in calculus, the absolute h

SUBMITTER: Ziesemer KA 

PROVIDER: S-EPMC6519167 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature