Top-Down Protein Identification using a Time-of-Flight Mass Spectrometer and Data Independent Acquisition.

ABSTRACT: Top-down mass spectrometry and direct dissociation of gas phase intact proteins have been demonstrated to be a powerful platform for identifying proteins from complex mixtures and for elucidating post-translational modifications (PTMs). Fragmentation of proteins in the atmospheric pressure/vacuum interface of the electrospray ionization mass spectrometer is an effective dissociation technique that can be utilized for on-line HPLC top-down analysis. We demonstrate the capability to perform intact protein identifications in a single-stage time-of- flight (TOF) mass spectrometer in a data independent (DIA) acquisition fashion by rapidly switching the in-source dissociation (ISD) energy during protein elution from a liquid chromatography (LC) column. The intact protein and product ion masses o

SUBMITTER: Lakshmanan R 

PROVIDER: S-EPMC6519736 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature