Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro.

ABSTRACT:

Objective

Aims were to investigate sensitivity of various human and canine cancer cell lines to hyperthermia and the influence of particular treatment conditions, and to analyze the DNA-damage response and mode of cell death in cell line radiosensitized by hyperthermia. Additionally, we were interested in the involvement of HSP70 in radiosensitization.

Methods

Radiosensitization by hyperthermia was determined in a panel of human and canine cancer cell lines using clonogenic cell survival assay, as well as levels of heat shock proteins (HSPs) using immunoblotting. The influence of the hyperthermia-radiotherapy time gap, different temperatures and the order of treatments on clonogenicity of hyperthermia-sensitive A549 cells was investigated. Additionally, DNA damage and cell d

SUBMITTER: Nytko KJ 

PROVIDER: S-EPMC6519812 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature