Machine Learning Based Real-Time Image-Guided Cell Sorting and Classification.

ABSTRACT: Cell classification based on phenotypical, spatial, and genetic information greatly advances our understanding of the physiology and pathology of biological systems. Technologies derived from next generation sequencing and fluorescent activated cell sorting are cornerstones for cell- and genomic-based assays supporting cell classification and mapping. However, there exists a deficiency in technology space to rapidly isolate cells based on high content image information. Fluorescence-activated cell sorting can only resolve cell-to-cell variation in fluorescence and optical scattering. Utilizing microfluidics, photonics, computation microscopy, real-time image processing and machine learning, we demonstrate an image-guided cell sorting and classification system possessing the high throughput

SUBMITTER: Gu Y 

PROVIDER: S-EPMC6520141 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature