Dipolar DC induced collisional activation of non-dissociated electron-transfer products.

ABSTRACT: The application of electron transfer and dipolar direct current induced collisional activation (ET-DDC) for enhanced sequence coverage of peptide/protein cations is described. A DDC potential is applied across one pair of opposing rods in the high-pressure collision cell of a hybrid quadrupole/time-of-flight tandem mass spectrometer (QqTOF) to induce collisional activation, in conjunction with electron transfer reactions. As a broadband technique, DDC can be employed for the simultaneous collisional activation of all the first-generation charge-reduced precursor ions (eg, electron transfer no-dissociation or ETnoD products) from electron transfer reactions over a relatively broad mass-to-charge range. A systematic study of ET-DDC induced collision activation on peptide/protein cations reve

SUBMITTER: Adhikari S 

PROVIDER: S-EPMC6520196 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature