Lysosomotropic challenge of mast cells causes intra-granular reactive oxygen species production.

ABSTRACT: Mast cells contribute to the pathology of allergic and other disorders. Strategies to interfere with harmful mast cell-related activities are therefore warranted. Previously we established a principle for inducing selective apoptosis of mast cells, by the use of lysosomotropic agents that cause secretory granule permeabilization, leading to production of reactive oxygen species (ROS). However, the mechanism of ROS production has not been known. Here we addressed this issue. Live microscopy analysis showed that the secretory granules comprise major subcellular compartments for ROS production in response to mefloquine. As further signs for the primary involvement of secretory granules, both ROS production and cell death was blunted in mast cells lacking serglycin, a secretory granule-restric

SUBMITTER: Paivandy A 

PROVIDER: S-EPMC6520368 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature