Generation of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptor.

ABSTRACT: Invariant natural killer T (iNKT) cells are a specialized subset of T cells contributing to both, the innate and adaptive immune responses. In contrast to conventional T lymphocytes they recognize lipid antigens. The aim of the project is to establish a novel model system, to study iNKT-TCR - ligand interaction. An iNKT reporter cell line (JE6-1^REP-iNKT) was engineered by introducing the human iNKT-TCR into a human leukemic T cell line carrying an NF-κB-driven fluorescent transcriptional reporter construct. Antigen presenting BW^STIM cells expressing human CD1d and CD80 were generated. Reporter induction in JE6-1^REP-iNKT cells was assessed by flow cytometry. CRISPR/Cas9 was used for β2M knock out in JE6-1^REP-iNKT cells to abrogate CD1d expression

SUBMITTER: Humeniuk P 

PROVIDER: S-EPMC6520406 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature